The repository is available online (www.rbwb.org; please see Neurotransporter Atlas: VGLUT3). In line with the policy of the University of Oslo, access is not monitored.
Vesicular glutamate transporter-3 in the rodent brain: Vesicular colocalization with vesicular γ-aminobutyric acid transporter
Article first published online: 17 JUL 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 521, Issue 13, pages 3042–3056, 1 September 2013
How to Cite
Stensrud, M.J., Chaudhry, F.A., Leergaard, T.B., Bjaalie, J.G. and Gundersen, V. (2013), Vesicular glutamate transporter-3 in the rodent brain: Vesicular colocalization with vesicular γ-aminobutyric acid transporter. J. Comp. Neurol., 521: 3042–3056. doi: 10.1002/cne.23331
- Issue published online: 17 JUL 2013
- Article first published online: 17 JUL 2013
- Accepted manuscript online: 30 APR 2013 08:31AM EST
- Manuscript Accepted: 13 MAR 2013
- Manuscript Revised: 8 MAR 2013
- Manuscript Received: 9 NOV 2012
- Research Council of Norway. Grant Number: 170441/V40
- Helse Sør-Øst, Norway. Grant Number: 6051–10920-39130
Vesicular glutamate transporters (VGLUT1–3) carry glutamate into synaptic vesicles. VGLUT3 has been reported to be localized in nonglutamatergic neuronal populations in the brain. However, detailed subcellular localization of VGLUT3 has not been shown. In particular, the identity of synaptic vesicles expressing VGLUT3 remains to be revealed. Here we present novel electron microscopic postembedding immunogold data from mouse and rat brains showing that small, clear, and round synaptic vesicles in γ-aminobutyric acid (GABA)-ergic nerve terminals contain labeling for both VGLUT3 and the vesicular GABA transporter (VGAT). Immunoisolation of synaptic vesicles confirmed the immunogold data and showed vesicular colocalization of VGLUT3 and VGAT. Moreover, we show that gold particles signaling VGLUT3 are present in synaptic vesicles in acetylcholinergic nerve terminals in the striatum. Quantitative immunogold analyses reveal that the density of VGLUT3 gold particles is similar in GABAergic terminals in the hippocampus and the neocortex to that in cholinergic terminals in the striatum. In contrast to in the hippocampus and the neocortex, VGLUT3 was absent from VGAT-positive terminals in the striatum. The labeling pattern produced by the VGLUT3 antibodies was found to be specific; there was no labeling in VGLUT3 knockout tissue, and the observed labeling throughout the rat brain corresponds to the known light-microscopic distribution of VGLUT3. From the present results, we infer that glutamate is released with GABA from inhibitory terminals and acetylcholine from cholinergic terminals. J. Comp. Neurol. 521: 3042–3056, 2013. © 2013 Wiley Periodicals, Inc.