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Diverse neuronal lineages make stereotyped contributions to the Drosophila locomotor control center, the central complex

Authors

  • Jacob S. Yang,

    1. Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 20147
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    • The first three authors contributed equally to this study.

  • Takeshi Awasaki,

    1. Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 20147
    Current affiliation:
    1. Kyorin University School of Medicine, Tokyo, Japan
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    • The first three authors contributed equally to this study.

  • Hung-Hsiang Yu,

    1. Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 20147
    Current affiliation:
    1. Institute of Cellular and Organismic Biology, Taipei, Taiwan
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    • The first three authors contributed equally to this study.

  • Yisheng He,

    1. Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, 01605
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  • Peng Ding,

    1. Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, 01605
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  • Jui-Chun Kao,

    1. Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, 01605
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  • Tzumin Lee

    Corresponding author
    1. Department of Neurobiology, University of Massachusetts, Worcester, Massachusetts, 01605
    • Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia, 20147
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Correspondence to: Tzumin Lee, Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA, 20147. E-mail: leetz@janelia.hhmi.org

Abstract

The Drosophila central brain develops from a fixed number of neuroblasts. Each neuroblast makes a clone of neurons that exhibit common trajectories. Here we identified 15 distinct clones that carry larval-born neurons innervating the Drosophila central complex (CX), which consists of four midline structures including the protocerebral bridge (PB), fan-shaped body (FB), ellipsoid body (EB), and noduli (NO). Clonal analysis revealed that the small-field CX neurons, which establish intricate projections across different CX substructures, exist in four isomorphic groups that respectively derive from four complex posterior asense-negative lineages. In terms of the region-characteristic large-field CX neurons, we found that two lineages make PB neurons, 10 lineages produce FB neurons, three lineages generate EB neurons, and two lineages yield NO neurons. The diverse FB developmental origins reflect the discrete input pathways for different FB subcompartments. Clonal analysis enlightens both development and anatomy of the insect locomotor control center. J. Comp. Neurol. 521:2645–2662, 2013. © 2013 Wiley Periodicals, Inc.

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