The first three authors contributed equally to this work.
Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum
Version of Record online: 25 JUL 2013
© 2013 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 521, Issue 14, pages 3303–3320, 1 October 2013
How to Cite
Juarez, M., Reyes, M., Coleman, T., Rotenstein, L., Sao, S., Martinez, D., Jones, M., Mackelprang, R. and De Bellard, M. E. (2013), Characterization of the trunk neural crest in the bamboo shark, Chiloscyllium punctatum. J. Comp. Neurol., 521: 3303–3320. doi: 10.1002/cne.23351
- Issue online: 25 JUL 2013
- Version of Record online: 25 JUL 2013
- Accepted manuscript online: 3 MAY 2013 02:33AM EST
- Manuscript Accepted: 25 APR 2013
- Manuscript Revised: 15 APR 2013
- Manuscript Received: 12 NOV 2012
- National Institute of Neurological Disorders and Stroke, National Institutes of Health . Grant Numbers: 2R15NS060099-02A1 , 5SC3GM096904-02 (to M.E.dB.)
- National Institutes of Health . Grant Number: GM 2 T34 GM008959 (to M.E. Zavala for support for M.J.)
- shark embryo;
- neural crest;
- Chiloscyllium punctatum
The neural crest is a population of mesenchymal cells that after migrating from the neural tube gives rise to structure and cell types: the jaw, part of the peripheral ganglia, and melanocytes. Although much is known about neural crest development in jawed vertebrates, a clear picture of trunk neural crest development for elasmobranchs is yet to be developed. Here we present a detailed study of trunk neural crest development in the bamboo shark, Chiloscyllium punctatum. Vital labeling with dioctadecyl tetramethylindocarbocyanine perchlorate (DiI) and in situ hybridization using cloned Sox8 and Sox9 probes demonstrated that trunk neural crest cells follow a pattern similar to the migratory paths already described in zebrafish and amphibians. We found shark trunk neural crest along the rostral side of the somites, the ventromedial pathway, the branchial arches, the gut, the sensory ganglia, and the nerves. Interestingly, C. punctatum Sox8 and Sox9 sequences aligned with vertebrate SoxE genes, but appeared to be more ancient than the corresponding vertebrate paralogs. The expression of these two SoxE genes in trunk neural crest cells, especially Sox9, matched the Sox10 migratory patterns observed in teleosts. Also of interest, we observed DiI cells and Sox9 labeling along the lateral line, suggesting that in C. punctatum, glial cells in the lateral line are likely of neural crest origin. Although this has been observed in other vertebrates, we are the first to show that the pattern is present in cartilaginous fishes. These findings demonstrate that trunk neural crest cell development in C. punctatum follows the same highly conserved migratory pattern observed in jawed vertebrates. J. Comp. Neurol. 521:3303–3320, 2013. © 2013 Wiley Periodicals, Inc.