Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain
Article first published online: 25 JUL 2013
© 2013 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 521, Issue 14, pages 3340–3357, 1 October 2013
How to Cite
Shinohara, M., Zhu, Y. and Murakami, F. (2013), Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain. J. Comp. Neurol., 521: 3340–3357. doi: 10.1002/cne.23353
- Issue published online: 25 JUL 2013
- Article first published online: 25 JUL 2013
- Accepted manuscript online: 3 MAY 2013 02:32AM EST
- Manuscript Accepted: 25 APR 2013
- Manuscript Revised: 9 APR 2013
- Manuscript Received: 8 JAN 2013
- Ministry of Education, Culture, Sports, Science & Technology . Grant Number: Grant-in-Aid for Scientific Research, Kakenhi, 22220004
Additional Supporting Information may be found in the online version of this article.
|cne23353-sup-0001-suppinfo.mov||34357K||Supplementary Movie 1. Assembly of four time-lapse sequences showing the time course of neuronal movement within the PN of E12.5-labeled neurons. At E15.5, actively moving cells were present throughout the nuclear region. At E16.5 and E17.5, moving cells become mostly restricted to the ventral half of the PN. Finally at E18.5, the majority of cells appeared stationary. This movie corresponds to Figure 1 (H-K). Scale bar: 50 µm.|
|cne23353-sup-0002-suppinfo.mov||15892K||Supplementary Movie 2. Assembly of two time-lapse sequences showing the time course of neuronal movement within the PN of E14.5-labeled neurons. While many cells were motile at E17.5, most became stationary at E18.5. This movie corresponds to Figure 1 (L-M). Scale bar: 50 µm.|
|cne23353-sup-0003-suppinfo.mov||24977K||Supplementary Movie 3. Time-lapse observations of early- and late-born PN neurons show a reduced tendency of late-borns to turn radially within the PN. A notable portion of the early-born PN neurons turned to migrate radially within the PN at E15.5, but only few late-borns did at E17.5. Magenta arrowheads indicate radially migrating cells. This movie corresponds to Figure 3 (A-B). Scale bar: 100 µm.|
|cne23353-sup-0004-suppinfo.mov||20078K||Supplementary Movie 4. A time-lapse sequence showing a typical example of a PN neuron reversing its migration from the medial to lateral directions. The neuron, indicated by the arrow, initially migrated towards the midline with a medially-directed leading process, but reversed to migrate away from the midline with a laterally-directed leading process appearing de novo (indicated by the asterisk) This movie corresponds to Figure 4 (A). Scale bar: 20 µm.|
|cne23353-sup-0005-suppinfo.mov||13526K||Supplementary Movie 5. Two time-lapse sequences showing laterally migrating E12.5-labeled PN neurons at E15.5 and E17.5. The laterally migrating cells are marked by magenta arrows. More cells appear to migrate laterally at E17.5. This movie corresponds to Figure 4 (C, D). Scale bar: 50 µm|
|cne23353-sup-0006-suppinfo.mov||16654K||Supplementary Movie 6. A PN neuron showing a reversal of migration near the midline (dashed line). The reversal is followed by midline crossing of the leading process tip. Arrows indicate the cell soma of the migrating neuron and arrowheads indicate the tip of the leading process. Images were captured from an E15.5 slice. Scale bar: 100 µm.|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.