Novel variant of neuronal intranuclear rodlet immunoreactive for 40 kDa huntingtin associated protein and ubiquitin in the mouse brain
Article first published online: 25 SEP 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 521, Issue 16, pages 3832–3846, November 2013
How to Cite
Milman, P. and Woulfe, J. (2013), Novel variant of neuronal intranuclear rodlet immunoreactive for 40 kDa huntingtin associated protein and ubiquitin in the mouse brain. J. Comp. Neurol., 521: 3832–3846. doi: 10.1002/cne.23381
- Issue published online: 25 SEP 2013
- Article first published online: 25 SEP 2013
- Accepted manuscript online: 8 JUN 2013 01:51AM EST
- Manuscript Accepted: 23 MAY 2013
- Manuscript Revised: 11 APR 2012
- Manuscript Received: 5 JAN 2012
- Physicians Services Incorporated Foundation Inc.
- Ottawa Hospital Pathology and Laboratory Medicine (PALM) Research Fund
- rodlet of Roncoroni;
- locus coeruleus;
- nuclear bodies;
- melanin concentrating hormone
Intranuclear rodlets (INRs), also known as rodlets of Roncoroni, are poorly understood intranuclear bodies originally identified within neuronal nuclei on the basis of their unique morphology. The mechanisms of their formation, their biochemical composition and their physiological significance remain unknown. Using double immunofluorescence staining of mouse brain sections, we identified a novel variant of INR that is immunoreactive for the 40 kDa huntingtin associated protein (Hap40) and ubiquitin, and provide evidence for the existence of additional INR subtypes sharing ubiquitin immunoreactivity as a common feature. We describe a selective association of these INRs with melanin concentrating hormone (MCH) and tyrosine hydroxylase immunoreactive neurons of the hypothalamus and the locus coeruleus, respectively. We also demonstrate for the first time that biochemically distinct INR subtypes can coexist within a single nucleus where they engage in nonrandom spatial interactions. Our findings highlight the biochemical diversity and cell type-specific expression of these enigmatic intranuclear structures. J. Comp. Neurol. 521:3832–3846, 2013. © 2013 Wiley Periodicals, Inc.