The first two authors contributed equally to this work.
Neuropeptidome of Tribolium castaneum antennal lobes and mushroom bodies
Article first published online: 6 DEC 2013
Copyright © 2013 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 522, Issue 2, pages 337–357, 01 February 2014
How to Cite
Binzer, M., Heuer, C. M., Kollmann, M., Kahnt, J., Hauser, F., Grimmelikhuijzen, C. J.P. and Schachtner, J. (2014), Neuropeptidome of Tribolium castaneum antennal lobes and mushroom bodies. J. Comp. Neurol., 522: 337–357. doi: 10.1002/cne.23399
- Issue published online: 6 DEC 2013
- Article first published online: 6 DEC 2013
- Accepted manuscript online: 1 JUL 2013 12:27PM EST
- Manuscript Accepted: 19 JUN 2013
- Manuscript Revised: 27 MAY 2013
- Manuscript Received: 23 NOV 2012
- Deutsche Forschungsgemeinschaft - priority program “Integrative analysis of olfaction” (SPP 1392) . Grant Number: SCHA 678/13-2
- mass spectrometry;
- olfactory system;
Neuropeptides are a highly diverse group of signaling molecules that affect a broad range of biological processes in insects, including development, metabolism, behavior, and reproduction. In the central nervous system, neuropeptides are usually considered to act as neuromodulators and cotransmitters that modify the effect of “classical” transmitters at the synapse. The present study analyzes the neuropeptide repertoire of higher cerebral neuropils in the brain of the red flour beetle Tribolium castaneum. We focus on two integrative neuropils of the olfactory pathway, the antennal lobes and the mushroom bodies. Using the technique of direct peptide profiling by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, we demonstrate that these neuropils can be characterized by their specific neuropeptide expression profiles. Complementary immunohistological analyses of selected neuropeptides revealed neuropeptide distribution patterns within the antennal lobes and the mushroom bodies. Both approaches revealed consistent differences between the neuropils, underlining that direct peptide profiling by mass spectrometry is a fast and reliable method to identify neuropeptide content. J. Comp. Neurol. 522:337–357, 2014. © 2013 Wiley Periodicals, Inc.