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cne23471-sup-0001-suppfig1.tif7045KSupplementary Figure 1. Photomicrographs of sagittal (A,E,G) and transverse (B-D, F) sections through the mammillary and tuberal hypothalamus of Xenopus, showing the expressions of xShh (A), Nkx2.1 (B), and Isl1 (C) and the combined expressions of Isl1 and Nkx2.1 (D,E), Isl1 and Otp (F), Nkx2.1 and Nkx2.2 (G) and Pax7 and Nkx2.1 (H). Developmental stages are indicated in each photograph. The schematic representation at the bottom summarizes the boundaries between the different basal hypothalamic subdivisions and the combinatorial code of markers of each region. The yellow lines indicate the limit between two adjacent regions revealed by the different markers. Scales bars: 100μm (A, F-H), 50μm (E). The scale bar in b is valid for C and D = 100μm. This figure corresponds to the figure 2.
cne23471-sup-0002-suppfig2.tif11953KSupplementary Figure 2. Photomicrographs of transverse (A-E,G, H) and sagittal (F, I-K) sections through the hypothalamus of Xenopus showing in the tuberal region and adjacent areas the expressions of Nkx2.1 (A) and Isl1 (B), and the combined expressions of Isl1 and Nkx2.1 (C), xShh and Isl1 (D), Isl1 and Otp (E,F), Nkx2.2 and Nkx2.1 (G), Nkx2.2 and otp (H,I), Nkx2.2 and SOM (J), and xLhx1 and Nkx2.2 (K). Developmental stages are indicated in each photograph. The schematic representations at the bottom summarize the combinatorial codes of the markers in the tuberal hypothalamus in a lateral view of the brain and a transverse section. Scale bars: 100μm. The scale bar in A is valid for B and C. This figure corresponds to the figure 3.
cne23471-sup-0003-suppfig3.tif11863KSupplementary Figure 3. Photomicrographs of transverse (A, C-F, H, J) and sagittal (B, G, I) sections through the developing hypothalamus of Xenopus showing in the mammillary region and adjacent areas the combined expression of Isl1 and Nkx2.1 (A,B), xShh and Nkx2.1 (C), Isl1 and Otp (D, E, F), Nkx2.2 and Otp (G I, J), Nkx2.2 and Nkx2.1 (H). The level of the transverse section shown in J is indicated by the vertical yellow line drawn in I. Developmental stages are indicated in each photograph. Scale bars: 100μm. This figure corresponds to the figure 4.
cne23471-sup-0004-suppfig4.tif15431KSupplementary Figure 4. Photomicrographs of sagittal (A, E-I) and transverse (B-D) sections through the developing hypothalamus of Xenopus showing in the mammillary region and adjacent areas the combined expression of Nkx2.2 and Nkx2.1 (A-C), xDll4 and Isl1 (D), xDll4 and Otp (E), xLhx1 and Otp (F). Single expression for xLhx1 (G) and Nkx2.2 (H) are shown together with the merged image (I). Developmental stages are indicated in each photograph. Scale bars: 100μm. The scale bar in G is valid for H and I. This figure corresponds to the figure 5.
cne23471-sup-0005-suppfig5.tif11485KSupplementary Figure 5. Photomicrographs of transverse (A, B, D-K) and sagittal (C,L) sections through the developing hypothalamus of Xenopus showing in the mammillary region and adjacent areas the combined expressions of Pax7 and Nkx2.1 (A, D) and Pax7 and Otp (E). The arrowheads in A, D, E and F show double labeled cells. In addition, imagines are shown of the combined labelling for TH with Nkx2.1 (F), xShh (G), Isl1 (H), Otp (I), xLhx1 (J) and Nkx2.2 (K,L). The schematic representations at the bottom summarize the combinatorial codes of the markers in the mammillary hypothalamus in a lateral view of the brain and a transverse section. Scale bars: 100μm (A, B, E-L), 50μm (C). The scale bar in E is valid for D. This figure corresponds to the figure 6.
cne23471-sup-0006-suppfig6.tif19822KSupplementary Figure 6. Photomicrographs of sagittal (A) and transverse (B-N) sections showing the retrogradely labeled cells and anterogradely labeled fibers after BDA injection in the tuberal region of juvenile Xenopus, combined with the marker Isl1 (A,C,E,H-J, L) to corroborate that the tracer was delivered into this paricular area. The dashed box in C,E and K indicate the higher magnification shown in C',E' and K', respectively. The arrowheads in A,B,C',E',J,M and n point to retrogradely labeled cells or fibers. Scale bars: 500μm (A), 100 μm (C-N), 50 μm (B, K'), 25 μm (C', E'). This figure corresponds to the figure 8.
cne23471-sup-0007-suppfig7.tif14483KSupplementary Figure 7. Photomicrographs of transverse sections showing the retrogradely labeled cells and anterogradely labeled fibers after BDA injection in the Ma of juvenile Xenopus, combined with the markers Isl1 (B-I) and Otp (D) to corroborate that the tracer was delivered into this particular area. The arrowheads in b-j indicate retrogradely labeled cells. Sclae bars: 200 μm (G-I, J'), 100 μm (A-F, K). This figure corresponds to the figure 9.

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