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cne23544-sup-0001-suppmovie1.mov9461KMovie A. The ventral half of the r1-r7 hindbrain region of the specimen in Figures 1A and 2 is displayed in 20 serial horizontal sections showing neurons retrogradely labeled with biotin dextran 10K from bilateral application to the ventral, rostral spinal cord. Sections were cut at 60 micrometer thickness, processed to yield black reaction product from the label, and counterstained with Cresyl Violet (see methods). The series runs from dorsal to ventral in the movie and rostral is towards the top of the frame. Rigid body registration of the slices was performed using the Stackreg plugin for ImageJ written by P. Thévenaz (
cne23544-sup-0002-suppmovie2.mov32901KMovie B. 3D Stereo rotations of maximum value projections (ImgeJ) of the sections shown in movie A with the pixel values inverted, i.e., the dark reaction product appears bright, the background dark. The blue channel of the RGB section stack was used for the projections, which span +/- 20 degrees around the Y-axis and are offset by 10 degrees between the left and right images. The fused image will appear as a dorsal view for crossed gaze and as a ventral view for parallel/divergent gaze. Note that while the reconstructions give the appearance of being tomographic they are projections based on images of discrete thick sections and are thus intended to give essentially macroscopic views of hindbrain neuronal organization.
cne23544-sup-0003-suppmovie3.mov36393KMovie C. The same projections as in Movie B, but with left and right images switched, giving a ventral view for crossed gaze and a dorsal view for parallel/divergent gaze.

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