A hindbrain segmental scaffold specifying neuronal location in the adult goldfish, Carassius auratus
Article first published online: 19 APR 2014
Copyright © 2014 Wiley Periodicals, Inc.
Journal of Comparative Neurology
Volume 522, Issue 10, pages 2446–2464, 1 July 2014
How to Cite
Gilland, E., Straka, H., Wong, T.W., Baker, R. and Zottoli, S.J. (2014), A hindbrain segmental scaffold specifying neuronal location in the adult goldfish, Carassius auratus. J. Comp. Neurol., 522: 2446–2464. doi: 10.1002/cne.23544
- Issue published online: 19 APR 2014
- Article first published online: 19 APR 2014
- Accepted manuscript online: 22 JAN 2014 11:08AM EST
- Manuscript Accepted: 17 JAN 2014
- Manuscript Revised: 15 JAN 2014
- Manuscript Received: 7 JUN 2013
- Deutsche Forschungsgemeinschaft . Grant Number: SFB 870, TP B12 (to H.S.)
- Essel Foundation (to S.J.Z.)
Additional Supporting Information may be found in the online version of this article.
|cne23544-sup-0001-suppmovie1.mov||9461K||Movie A. The ventral half of the r1-r7 hindbrain region of the specimen in Figures 1A and 2 is displayed in 20 serial horizontal sections showing neurons retrogradely labeled with biotin dextran 10K from bilateral application to the ventral, rostral spinal cord. Sections were cut at 60 micrometer thickness, processed to yield black reaction product from the label, and counterstained with Cresyl Violet (see methods). The series runs from dorsal to ventral in the movie and rostral is towards the top of the frame. Rigid body registration of the slices was performed using the Stackreg plugin for ImageJ written by P. Thévenaz (http://bigwww.epfl.ch/thevenaz/stackreg/).|
|cne23544-sup-0002-suppmovie2.mov||32901K||Movie B. 3D Stereo rotations of maximum value projections (ImgeJ) of the sections shown in movie A with the pixel values inverted, i.e., the dark reaction product appears bright, the background dark. The blue channel of the RGB section stack was used for the projections, which span +/- 20 degrees around the Y-axis and are offset by 10 degrees between the left and right images. The fused image will appear as a dorsal view for crossed gaze and as a ventral view for parallel/divergent gaze. Note that while the reconstructions give the appearance of being tomographic they are projections based on images of discrete thick sections and are thus intended to give essentially macroscopic views of hindbrain neuronal organization.|
|cne23544-sup-0003-suppmovie3.mov||36393K||Movie C. The same projections as in Movie B, but with left and right images switched, giving a ventral view for crossed gaze and a dorsal view for parallel/divergent gaze.|
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