Localization of glycine-containing neurons in the Macaca monkey retina

Authors

  • Anita E. Hendrickson,

    1. Department of Biological Structure, Wayne State University School of Medicine, Detroit, Michigan 48201
    2. Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan 48201
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  • Margaret A. Koontz,

    1. Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan 48201
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  • Roberta G. Pourcho,

    1. University of Washington School of Medicine, Seattle, Washington 98195; and Department of Anatomy, Wayne State University School of Medicine, Detroit, Michigan 48201
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  • P. Vijay Sarthy,

    1. Department of Ophthalmology, Wayne State University School of Medicine, Detroit, Michigan 48201
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  • Dennis J. Goebel

    1. University of Washington School of Medicine, Seattle, Washington 98195; and Department of Anatomy, Wayne State University School of Medicine, Detroit, Michigan 48201
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Abstract

Autoradiography following 3H-glycine (Gly) uptake and immunocytochemistry with a Gly-specific antiserum were used to identify neurons in Macaca monkey retina that contain a high level of this neurotransmitter. High-affinity uptake of Gly was shown to be sodium dependent whereas release of both endogenous and accumulated Gly was calcium dependent. Neurons labeling for Gly included 40-46% of the amacrine cells and nearly 40% of the bipolars. Synaptic labeling was seen throughout the inner plexiform layer (IPL) but with a preferential distribution in the inner half. Bands of labeled puncta occurred in S2, S4, and S5.

Both light and postembedding electron microscopic (EM) immunocytochemistry identified different types of amacrine and bipolar cell bodies and their synaptic terminals. The most heavily labeled Gly+ cell bodies typically were amacrine cells having a single, thick, basal dendrite extending deep into the IPL and, at the EM level, electron-dense cytoplasm and prominent nuclear infoldings. This cell type may be homologous with the Gly2 cell in human retina (Marc and Liu: J. Comp. Neurol. 232:241-260, ′85) and the AII/ Gly2 of cat retina (Famiglietti and Kolb: Brain Res. 84:293-300, ′75; Pourcho and Goebel: J. Comp. Neurol. 233:473-480, ′85a). Gly+ amacrines synapse most frequently onto Gly amacrines and both Gly and Gly+ bipolars. Gly+ bipolar cells appeared to be cone bipolars because their labeled dendrites could be traced only to cone pedicles. The pattern of these labeled dendritic trees indicated that both diffuse and midget types of bipolars were Gly+. The EM distribution of labeled synapses showed Gly+ amacrine synapses throughout the IPL, but these composed only 11-23% of the amacrine population. Most of the Gly+ bipolar terminals were in the inner IPL, where 70% of all bipolar terminals were labeled. These findings are consistent with previous data from cats and humans and suggest that both amacrine and bipolar cells contribute to glycine-mediated neurotransmission in the monkey retina.

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