Compartment of brain-type cretine kinase and ubiquitous mitochondrial cretine kinase in neurons: Evidence for a cretine phosphate energy shuttle in adult rat brain
Version of Record online: 9 OCT 2004
Copyright © 1994 Wiley-Liss, Inc.
Journal of Comparative Neurology
Volume 343, Issue 3, pages 500–511, 15 May 1994
How to Cite
Friedman, D. L. and Roberts, R. (1994), Compartment of brain-type cretine kinase and ubiquitous mitochondrial cretine kinase in neurons: Evidence for a cretine phosphate energy shuttle in adult rat brain. J. Comp. Neurol., 343: 500–511. doi: 10.1002/cne.903430311
- Issue online: 9 OCT 2004
- Version of Record online: 9 OCT 2004
- Manuscript Accepted: 3 DEC 1993
- energy metabolism;
- synaptic transmission;
Multiple isoforms of cretine kinase (CK) are expressed in specific cell types as part of an energy delivery or shuttle system. To test hypothesis that neurons utilize a cretine phosphate energy shuttle, we examined the pattern of CK isoform expression and localization in adult rat brain. Two isoforms of CK are present in brain extracts, “brain-type,” or BCK, and the ubiquitin form of the mitochondrial CK (uMtCK), as detected by enzyme activity following nondenaturing electrophoresis and by Western blotting following denaturing electrophorsis. In formalin-fixed and parafinn-embedded sections of rat brain, uMtCK immunostaining is detected in the somata of all Golgi type I neurons in the cerebellum, pontine reticular formation, red nucleus, hippocampus, and cerebral cortex. Immunostainig for uMtCK appears throughout the cell body but not in nuclei. BCK immunostaining is also present in somata of Golgi type I neurons in the cerebellum, red nucleus, and pons and is distributed throughout the cell body and within nuclei. BCK immunostaining also appeared in neuronal processes and is concentrated in the molecular layers of the cerebellum and the hippocampus and in cortical pyramidal cell dendrites. These results demonstrate a coordinate pattern of expression and compartmentation of BCK and MtCK isoform in neurons, which provides an anatomic basis for the transfer of metabolic energy via a cretine phosphate energy shuttle. © 1994 Wiley-Liss, Inc.