Triple FRET: A tool for Studying Long-Range Molecular Interactions

Authors

  • Elke Haustein,

    1. Experimental Biophysics Group, MPI for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax:(+49) 551 – 201 1435
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  • Michael Jahnz,

    1. Experimental Biophysics Group, MPI for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax:(+49) 551 – 201 1435
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  • Petra Schwille Prof. Dr.

    1. Experimental Biophysics Group, MPI for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany, Fax:(+49) 551 – 201 1435
    2. BioTEC, TU Dresden, c/o MPI for Cell Biology and Molecular Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany, Fax: (49) 351-210-1409
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Abstract

original image

Molecular traffic lights: A FRET cascade involving three spectrally suitable dyes (see picture) is not only feasible, but the effective distance can easily be increased to 100 Å and beyond; this was previously inaccessible with conventional FRET measurements.

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