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Communication
Three-Color Single-Molecule Fluorescence Resonance Energy Transfer
Article first published online: 13 DEC 2004
DOI: 10.1002/cphc.200400261
Copyright © 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Clamme, J.-P. and Deniz, A. A. (2005), Three-Color Single-Molecule Fluorescence Resonance Energy Transfer. ChemPhysChem, 6: 74–77. doi: 10.1002/cphc.200400261
Publication History
- Issue published online: 7 JAN 2005
- Article first published online: 13 DEC 2004
- Manuscript Revised: 29 SEP 2004
- Manuscript Received: 2 JUN 2004
Keywords:
- DNA structures;
- fluorescence;
- FRET (fluorescence resonance energy transfer);
- single-molecule studies
A molecular ruler: A three-color single-molecule fluorescence resonance energy transfer (FRET) method is presented (see Figure inset), which can be used to simultaneously measure multiple molecular distance changes during molecular conformational changes and binding. The ability to directly study conformational subpopulations in a mixture of molecules with different interdye distances is highlighted by the well-separated peaks in the two- dimensional histogram in the Figure.