Article

Oxygen Microscopy by Two-Photon-Excited Phosphorescence

  1. Olga S. Finikova Dr.1,
  2. Artem Y. Lebedev Dr.1,
  3. Alexey Aprelev Dr.2,
  4. Thomas Troxler Dr.3,
  5. Feng Gao Dr.3,
  6. Carmen Garnacho Dr.4,
  7. Silvia Muro Prof.4,
  8. Robin M. Hochstrasser Prof.3,
  9. Sergei A. Vinogradov Prof.1

Article first published online: 28 JUL 2008

DOI: 10.1002/cphc.200800296

Issue

ChemPhysChem

ChemPhysChem

Volume 9, Issue 12, pages 1673–1679, August 25, 2008

Additional Information

How to Cite

Finikova, O. S., Lebedev, A. Y., Aprelev, A., Troxler, T., Gao, F., Garnacho, C., Muro, S., Hochstrasser, R. M. and Vinogradov, S. A. (2008), Oxygen Microscopy by Two-Photon-Excited Phosphorescence. ChemPhysChem, 9: 1673–1679. doi: 10.1002/cphc.200800296

Author Information

  1. 1

    Department of Biochemistry and Biophysics, University of Pennsylvannia, Philadelphia PA 19104-6059 (USA), Fax: (+1) 215-573-3787

  2. 2

    Department of Physics, Drexel University, Philadelphia, PA 19104 (USA)

  3. 3

    Department of Chemistry and RLBL, University of Pennsylvannia, Philadelphia, PA 19104 (USA)

  4. 4

    Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104 (USA)

Publication History

  1. Issue published online: 15 AUG 2008
  2. Article first published online: 28 JUL 2008
  3. Manuscript Received: 21 MAY 2008

Funded by

  • NIH. Grant Numbers: EB007279, HL081273, P41-RR001348

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (821K)

Keywords:

  • dendrimer;
  • energy transfer;
  • oxygen;
  • phosphorescence;
  • porphyrin

Abstract

High-resolution images of oxygen distributions in microheterogeneous samples are obtained by two-photon laser scanning microscopy (2P LSM), using a newly developed dendritic nanoprobe with internally enhanced two-photon absorption (2PA) cross-section. In this probe, energy is harvested by a 2PA antenna, which passes excitation onto a phosphorescent metalloporphyrin via intramolecular energy transfer. The 2P LSM allows sectioning of oxygen gradients with near diffraction-limited resolution, and lifetime-based acquisition eliminates dependence on the local probe concentration. The technique is validated on objects with a priori known oxygen distributions and applied to imaging of pO2 in cells.

View Full Article (HTML) Get PDF (821K)