Three-Dimensional Structure and Thermal Stability Studies of DNA Nanostructures by Energy Transfer Spectroscopy



original image

Structural changes and stability of DNA nanoarchitectures including Y-shaped DNA (see picture), dendrimer-like DNA, and DNA hydrogels are investigated. The results demonstrate the feasibility and flexibility of FRET and NSET (Förster resonance/ nanometal surface- energy transfer) in determining difficult-to-obtain 3D structures and characterizing the thermal responses of DNA nanoarchitectures in real time.