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Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells

  1. Young Shik Shin1,2,†,
  2. Habib Ahmad1,†,
  3. Dr. Qihui Shi1,†,
  4. Dr. Hyungjun Kim3,4,
  5. Dr. Tod A. Pascal3,4,
  6. Prof. Rong Fan5,
  7. Prof. William A. Goddard III3,4,
  8. Prof. James R. Heath1

Article first published online: 16 AUG 2010

DOI: 10.1002/cphc.201000528

Issue

ChemPhysChem

ChemPhysChem

Volume 11, Issue 14, pages 3063–3069, October 4, 2010

Additional Information

How to Cite

Shin, Y. S., Ahmad, H., Shi, Q., Kim, H., Pascal, T. A., Fan, R., Goddard, W. A. and Heath, J. R. (2010), Chemistries for Patterning Robust DNA MicroBarcodes Enable Multiplex Assays of Cytoplasm Proteins from Single Cancer Cells. ChemPhysChem, 11: 3063–3069. doi: 10.1002/cphc.201000528

Author Information

  1. 1

    Division of Chemistry and Chemical Engineering, Nanosystems Biology Cancer Center, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125 (USA), Fax: (+1)626-395-2355

  2. 2

    Division of Engineering and Applied Science, Bioengineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125 (USA)

  3. 3

    Graduate School of EEWS, Korea Advanced Institute of Science Technology, Daejeon, 305-701(Republic of Korea)

  4. 4

    Materials and Process Simulation Center, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125 (USA)

  5. 5

    Department of Biomedical Engineering, Yale University, PO Box 208260, New Haven, CT 06520 (USA)

  1. †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 24 SEP 2010
  2. Article first published online: 16 AUG 2010
  3. Manuscript Received: 1 JUL 2010

Funded by

  • National Cancer Institute. Grant Number: 5U54 CA119347
  • Jean Perkins Foundation
  • NRF of Korea. Grant Number: R31–2008–000–10055–0

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Keywords:

  • microarrays;
  • microfluidics;
  • proteins;
  • single-cell studies;
  • surface chemistry
Thumbnail image of graphical abstract

The optimization of chemistries to enable the patterning of miniaturized DNA barcodes using microfluidics flow channels is described (see picture). Experiment and theory reveal that solvent mixtures in which counterions are strongly associated with the negatively charged DNA oligomers may be harnessed to produce high quality, high density DNA microarray patterns over a large area.

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