FRET Imaging by kt/kf

Authors

  • Dr. M. Julia Roberti,

    1. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria, Pabellón II/Piso 3, 1428 Buenos Aires (Argentina), Fax: (+54) 11-4576-3346
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  • Dr. Luciana Giordano,

    1. Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry am Fassberg 11, 37077 Göttingen (Germany), Fax: (+49) 551-2011467
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  • Dr. Thomas M. Jovin,

    Corresponding author
    1. Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry am Fassberg 11, 37077 Göttingen (Germany), Fax: (+49) 551-2011467
    • Laboratory of Cellular Dynamics, Max Planck Institute for Biophysical Chemistry am Fassberg 11, 37077 Göttingen (Germany), Fax: (+49) 551-2011467
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  • Dr. Elizabeth A. Jares-Erijman

    Corresponding author
    1. Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria, Pabellón II/Piso 3, 1428 Buenos Aires (Argentina), Fax: (+54) 11-4576-3346
    • Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CIHIDECAR, CONICET, Ciudad Universitaria, Pabellón II/Piso 3, 1428 Buenos Aires (Argentina), Fax: (+54) 11-4576-3346
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Errata

This article is corrected by:

  1. Errata: Corrigendum: FRET Imaging by kt/kf Volume 12, Issue 6, 1033, Article first published online: 8 April 2011

Abstract

original image

A FRET formalism in terms of the ratio between the rate constants for deactivation of the donor excited state by resonance energy transfer (kt) and by emission (kf) is introduced (see picture). The advantages of the approach are illustrated by FRET imaging of the donor/acceptor system consisting of co-aggregates of the amyloid protein alpha-synuclein labeled with the biarsenical FlAsH and quantum dots (605 nm) functionalized with alpha-synuclein.

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