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Preparation of Micrometer-Sized Free-Standing Membranes

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Abstract

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Snap, crackle and pop! A simple method to obtain solvent-free, free-standing membranes suspended over apertures with 2.5 μm in diameter is described. The membranes are obtained by bursting of giant unilamellar vesicles (GUV) on a holey surface (see picture). Membrane integrity after bursting is fostered by enhancing the electrostatic interaction of the holey surface and the lipids.

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