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Domain-Specific Folding Kinetics of Staphylococcal Nuclease Observed through Single-Molecule FRET in a Microfluidic Mixer

Authors

  • Dr. Zeyong Zhi,

    1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering and Bio-dynamic Optical Imaging Center, Peking University, Beijing 100871 (P. R. China), Fax: (+86) 10-62751708
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  • Dr. Pengcheng Liu,

    1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering and Bio-dynamic Optical Imaging Center, Peking University, Beijing 100871 (P. R. China), Fax: (+86) 10-62751708
    2. National Key Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing 100101 (P. R. China)
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  • Peng Wang,

    1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering and Bio-dynamic Optical Imaging Center, Peking University, Beijing 100871 (P. R. China), Fax: (+86) 10-62751708
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  • Prof. Dr. Yanyi Huang,

    1. College of Engineering, and Biodynamic Optical Imaging Center, Peking University, Beijing 100871 (P. R. China)
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  • Prof. Dr. Xin Sheng Zhao

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering and Bio-dynamic Optical Imaging Center, Peking University, Beijing 100871 (P. R. China), Fax: (+86) 10-62751708
    • Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Department of Chemical Biology, College of Chemistry and Molecular Engineering and Bio-dynamic Optical Imaging Center, Peking University, Beijing 100871 (P. R. China), Fax: (+86) 10-62751708
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  • FRET: Fluorescence Resonance Energy Transfer

Abstract

original image

Fast Folding: A combined single-molecule fluorescence resonance energy transfer (smFRET) and microfluidic mixing study reveals that the α-helical subdomain of staphylococcal nuclease (SNase) (see picture) folds about ten times faster than the β-sheet subdomain.

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