Intracellular Conformations of Human Telomeric Quadruplexes Studied by Electron Paramagnetic Resonance Spectroscopy

Authors

  • Mykhailo Azarkh,

    1. Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
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  • Vijay Singh,

    1. Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
    2. Current address: Department of Chemistry, Stanford University, Stanford, CA 94305 (USA)
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  • Oliver Okle,

    1. Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
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  • Prof. Dr. Daniel R. Dietrich,

    1. Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
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  • Prof. Dr. Jörg S. Hartig,

    Corresponding author
    1. Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
    • Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
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  • Dr. Malte Drescher

    Corresponding author
    1. Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
    • Department of Chemistry, Konstanz Research School Chemical Biology (KoRS-CB) and Zukunftskolleg, University of Konstanz, 78457 Konstanz (Germany), Fax: (+49) 7531-883139
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Abstract

original image

In-cell DEER (in-cell double electron electron resonance) is applied to investigate quadruplex formation of the human telomeric repeat d[AGGG(TTAGGG)3]. The initially unfolded DNA sequence forms a mixture of different quadruplex topologies upon injection into living cells. In addition, time-dependent distance measurements are carried out to monitor quadruplex folding.

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