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Keywords:

  • fluorescence anisotropy;
  • fluorescence microscopy;
  • FRET;
  • lipids;
  • proteins
Thumbnail image of graphical abstract

Homo-FRET, Förster resonance energy transfer between identical fluorophores, can be employed in microscopy to quantify cluster sizes as well as cluster size distributions in cells, as shown by H. C. Gerritsen et al. on p. 475.