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Keywords:

  • adsorption;
  • atomic force microscopy;
  • peptides;
  • surface plasmon resonance;
  • thermodynamics
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Multi-technique methods involving surface plasmon resonance spectroscopy and atomic force microscopy provide experimental data for the characterization of peptide adsorption on self-assembled monolayers. A comparative study is carried out in phosphate-buffered saline (PBS) and potassium phosphate-buffered (PPB) water to determine the influence of the salt concentration on the adsorption behavior (see figure; ΔG0ads: free energy of peptide adsorption, Fdes: force required for peptide desorption).