Peptide–Surface Adsorption Free Energy Comparing Solution Conditions Ranging from Low to Medium Salt Concentrations

Authors

  • Dr. Yang Wei,

    1. Bioengineering Department, Clemson University, 501 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634 (USA), Fax: (+1) 864-656-5552
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  • Aby A. Thyparambil,

    1. Bioengineering Department, Clemson University, 501 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634 (USA), Fax: (+1) 864-656-5552
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  • Prof. Dr. Robert A. Latour

    Corresponding author
    1. Bioengineering Department, Clemson University, 501 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634 (USA), Fax: (+1) 864-656-5552
    • Bioengineering Department, Clemson University, 501 Rhodes Engineering Research Center, Clemson University, Clemson, SC 29634 (USA), Fax: (+1) 864-656-5552
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Abstract

original image

Multi-technique methods involving surface plasmon resonance spectroscopy and atomic force microscopy provide experimental data for the characterization of peptide adsorption on self-assembled monolayers. A comparative study is carried out in phosphate-buffered saline (PBS) and potassium phosphate-buffered (PPB) water to determine the influence of the salt concentration on the adsorption behavior (see figure; ΔG0ads: free energy of peptide adsorption, Fdes: force required for peptide desorption).

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