A prism-based surface plasmon coupled emission (SPCE) imaging apparatus with a reverse Kretschmann (RK) configuration was developed and applied to dye-doped polymer films. Highly polarized, directional and enhanced fluorescence images were obtained. The angular distribution of the SPCE images was in accordance with the validated theoretical calculation performed using Fresnel equation. Prism-based SPCE imaging combined with microarray technology appears to be a promising platform for rapid and high-throughput analysis, especially for high-density arrays. We believe that prism-based SPCE imaging has potential applications in biochemical research.