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Keywords:

  • cellular membranes;
  • fluorescence correlation spectroscopy;
  • lipids;
  • monolayers;
  • protein binding

Abstract

Model membrane systems have become invaluable tools to investigate specific features of cellular membranes. Although a variety of different experimental assays does exist, many of them are rather complicated in their preparation and difficult in their practical realisation. Here, we propose a new simple miniaturised monolayer assay that can easily be combined with standard analytical techniques such as confocal fluorescence microscopy and fluorescence correlation spectroscopy (FCS).