Investigation of Sickle-Cell Haemoglobin Polymerisation under Electrochemical Control
Article first published online: 23 MAY 2013
© 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Special Issue: Photo- and Bioelectrochemistry
Volume 14, Issue 10, pages 2143–2148, July 22, 2013
How to Cite
Iqbal, Z., Li, M., McKendry, R., Horton, M. and Caruana, D. J. (2013), Investigation of Sickle-Cell Haemoglobin Polymerisation under Electrochemical Control. ChemPhysChem, 14: 2143–2148. doi: 10.1002/cphc.201300203
- Issue published online: 25 JUL 2013
- Article first published online: 23 MAY 2013
- Manuscript Received: 26 FEB 2013
- Funded Access
- oxygen depletion;
- protein polymerisation;
- sickle-cell anemia
We describe an electrochemistry-based technique to control and monitor the polymerisation of sickle-cell haemoglobin (HbS). The polymerisation was monitored as a change in turbidity during the depletion of oxygen in a small volume custom-built thin-layer electrochemical cell. The cell allowed the investigation of HbS polymerisation as a function of HbS concentration, temperature and solution pH. We confirm that the oxygen was efficiently depleted using finite-element modelling to accurately recreate the electrochemical thin-layer cell. Understanding the nucleation and growth of HbS polymerisation will provide a better understanding of the pathophysiology of sickle-cell disease in vivo, and thus help improve therapeutic strategies for this common and frequently disabling disorder.