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A His-Tagged Melanocarpus albomyces Laccase and its Electrochemistry upon Immobilisation on NTA-Modified Electrodes and in Conducting Polymer Films

Authors

  • Dr. Maciej Sosna,

    1. Chemistry, University of Southampton, Highfield, SO17 1BJ, Southampton (UK), Fax: (+44) 23 8059 3781
    2. Current address: Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, DK-8000, Aarhus C (Denmark)
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  • Dr. Harry Boer,

    1. VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT (Finland)
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  • Prof. Philip N. Bartlett

    Corresponding author
    1. Chemistry, University of Southampton, Highfield, SO17 1BJ, Southampton (UK), Fax: (+44) 23 8059 3781
    • Chemistry, University of Southampton, Highfield, SO17 1BJ, Southampton (UK), Fax: (+44) 23 8059 3781

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Abstract

The article describes the construction, immobilisation and electrochemistry of histidine tagged laccase from Melanocarpus albomyces. A facile method of functionalisation of glassy carbon electrodes with nitrilotriacetic acid (NTA) using diazonium grafting and solid state chemistry is described. NTA-modified electrodes are shown to bind laccase which reduces oxygen at neutral pH in the presence of soluble redox mediator. Laccase-modified electrodes are also prepared by enzyme immobilisation within poly(aniline)/poly(vinylsulfonate) films. The polymer is found to efficiently retain the enzyme as well as provide direct electrical contact between the electrode and the enzyme active centre. Cyclic voltammetry reveals the direct electron transfer to the enzyme is dependent on the redox state of the polymer film.

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