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Keywords:

  • cost-efficiency;
  • fluorescence;
  • laser spectroscopy;
  • localization microscopy;
  • super-resolution imaging
Thumbnail image of graphical abstract

Crystal clear: The authors introduce a miniaturized localization microscopy setup based on cost-effective components. They demonstrate its feasibility for subdiffraction resolution fluorescence imaging in resolving different cellular nanostructures. The setup can be used advantageously in practical courses for training students in super-resolution fluorescence microscopy.