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A Blueprint for Cost-Efficient Localization Microscopy

Authors

  • Thorge Holm,

    1. Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509
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  • Teresa Klein,

    1. Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509
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  • Anna Löschberger,

    1. Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509
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  • Tobias Klamp,

    1. Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509
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  • Dr. Gerd Wiebusch,

    1. Biomolecular Photonics, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld (Germany)
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  • Dr. Sebastian van de Linde,

    Corresponding author
    1. Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509
    • Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509

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  • Prof. Dr. Markus Sauer

    Corresponding author
    1. Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509
    • Department of Biotechnology & Biophysics, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg (Germany), Fax: (+49) 931-31-84509

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Abstract

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Crystal clear: The authors introduce a miniaturized localization microscopy setup based on cost-effective components. They demonstrate its feasibility for subdiffraction resolution fluorescence imaging in resolving different cellular nanostructures. The setup can be used advantageously in practical courses for training students in super-resolution fluorescence microscopy.

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