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Crowding Alters the Dynamics and the Length of RecA Nucleoprotein Filaments in RecA-Mediated Strand Exchange

Authors

  • Hung-Yi Wu,

    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C). Fax: (+886) 2-2363-6359
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  • Prof. Dr. Hung-Wen Li

    Corresponding author
    1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C). Fax: (+886) 2-2363-6359
    • Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C). Fax: (+886) 2-2363-6359

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Abstract

original image

Crowd impact: Molecular crowding effects of bovine serum albumin and poly(ethylene glycol) on the Escherichia coli RecA-mediated strand exchange reaction are quantified by using a single-molecule outgoing strand experiment and magnetic pull-down and ATPase assays. The alterations of the biochemical parameters of this complex enzymatic reaction in such crowded environments are discussed.

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