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Keywords:

  • DNA origami;
  • DNA PAINT;
  • fluorescence;
  • resolution;
  • superresolution microscopy
Thumbnail image of graphical abstract

To demonstrate resolution down to 6 nm with the superresolution technique DNA PAINT, binding sites for fluorescent dyes are placed at different distances on top of DNA origami nanostructures, analogous to a macroscopic vision test. P. Tinnefeld et al. show on p. 2431 that distances down to 6 nm can be resolved with DNA PAINT. DNA origami can be used to prove this resolution as many identical structures allow statistical evaluation.