Back Cover: Fluorescence Microscopy with 6 nm Resolution on DNA Origami (ChemPhysChem 12/2014)

Authors

  • Mario Raab,

    1. Institute for Physical and Theoretical Chemistry and Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Hans-Sommer Str. 10, 38106 Braunschweig (Germany)
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  • Jürgen J. Schmied,

    1. Institute for Physical and Theoretical Chemistry and Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Hans-Sommer Str. 10, 38106 Braunschweig (Germany)
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  • Ija Jusuk,

    1. Institute for Physical and Theoretical Chemistry and Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Hans-Sommer Str. 10, 38106 Braunschweig (Germany)
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  • Dr. Carsten Forthmann,

    1. Institute for Physical and Theoretical Chemistry and Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Hans-Sommer Str. 10, 38106 Braunschweig (Germany)
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  • Prof. Dr. Philip Tinnefeld

    Corresponding author
    1. Institute for Physical and Theoretical Chemistry and Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Hans-Sommer Str. 10, 38106 Braunschweig (Germany)
    • Institute for Physical and Theoretical Chemistry and Braunschweig Integrated Centre of Systems Biology (BRICS), Braunschweig University of Technology, Hans-Sommer Str. 10, 38106 Braunschweig (Germany)

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Abstract

original image

To demonstrate resolution down to 6 nm with the superresolution technique DNA PAINT, binding sites for fluorescent dyes are placed at different distances on top of DNA origami nanostructures, analogous to a macroscopic vision test. P. Tinnefeld et al. show on p. 2431 that distances down to 6 nm can be resolved with DNA PAINT. DNA origami can be used to prove this resolution as many identical structures allow statistical evaluation.

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