DCDHF Fluorophores for Single-Molecule Imaging in Cells (pages 55–65)
Samuel J. Lord, Nicholas R. Conley, Hsiao-lu D. Lee, Stefanie Y. Nishimura, Andrea K. Pomerantz, Katherine A. Willets, Zhikuan Lu, Hui Wang, Na Liu, Reichel Samuel, Ryan Weber, Alexander Semyonov, Meng He, Robert J. Twieg and W. E. Moerner
Version of Record online: 21 NOV 2008 | DOI: 10.1002/cphc.200800581
Push–pull fluorophores: The photophysics of DCDHF fluorophores (see figure) are tuned and measured for optimization for imaging in living cells. The fluorophores are comparatively photostable, exhibit sensitivity to their local environment, and can be re-engineered to introduce more sophisticated photophysics (e.g. photoactivation).