Improved Photon Yield from a Green Dye with a Reducing and Oxidizing System (pages 1657–1660)
Antoine Le Gall , Dr. David Dulin , Dr. Gilles Clavier, Dr. Rachel Méallet-Renault, Dr. Philippe Bouyer, Dr. Karen Perronet and Prof. Dr. Nathalie Westbrook
Article first published online: 31 MAY 2011 | DOI: 10.1002/cphc.201100085
An optimized chemistry turns a small green dye into a useful label for single-molecule experiments where steric hindrance is an issue. Thanks to a reducing and oxidizing system combined to oxygen depletion, a single Bodipy (boron-dipyrromethene) FL fluorophore emits, on average, 20 times more photons around 510 nm (see picture) and its lifetime before photobleaching is increased by the same amount, reaching several seconds.