Cover Picture: Activation of Integrin Function by Nanopatterned Adhesive Interfaces (ChemPhysChem 3/2004) (page 293)
Marco Arnold, Elisabetta Ada Cavalcanti-Adam, Roman Glass, Jacques Blümmel, Wolfgang Eck, Martin Kantlehner, Horst Kessler and Joachim P. Spatz
Article first published online: 10 MAR 2004 | DOI: 10.1002/cphc.200490013
The cover picture shows the first demonstration of cell adhesion activation through a nanoadhesive pattern with single integrin resolution. Scanning electron microscopy images nanoscopic 6-nm large Au particles as white dots, which are functionalized with cell ligands and organized in a square pattern. The free glass substrate area between the Au is covered with a biologically inert polymer, thereby avoiding protein or cell interactions with the glass. A few cell lamellipodia experience this environment and adhere entirely to the Au–nanoparticle pattern squares. The substrate forms a well-defined, rigid adhesion pattern where Au particles control integrin–integrin interactions in focal adhesions by their separation distance. A separation between single intergrins of ≥73 nm results in limited cell attachment and spreading, and dramatically reduces the formation of focal adhesion and actin stress fibers. The range of 58–73 nm is found to be a universal length scale for integrin clustering and activation, since these properties are shared by a variety of cultured cells. Find out more in the Communication by Spatz et al. on page 383.