Localization of Single Avidin–Biotin Interactions Using Simultaneous Topography and Molecular Recognition Imaging (pages 897–900)
Andreas Ebner, Ferry Kienberger, Gerald Kada, Cordula M. Stroh, Manfred Geretschläger, A. S. M. Kamruzzahan, Linda Wildling, W. Travis Johnson, Brian Ashcroft, Jeremy Nelson, Stuart M. Lindsay, Hermann J. Gruber and Peter Hinterdorfer
Article first published online: 22 APR 2005 | DOI: 10.1002/cphc.200400545
Two-for-one: The authors discuss a powerful new tool that, in a single dynamic force microscopy scan, maps the topography of a surface and simultaneously recognizes specific target sites (see figure). Topography and recognition images correlated nicely at a lateral resolution of a few nanometers. This clever technique has great potential for a broad range of applications involving the detection of particular binding sites in native biological systems.