Visualization of Intrinsically Disordered Regions of Proteins by High-Speed Atomic Force Microscopy (pages 1859–1866)
Atsushi Miyagi, Yasuo Tsunaka , Takayuki Uchihashi , Kouta Mayanagi , Susumu Hirose, Kosuke Morikawa and Toshio Ando
Article first published online: 12 AUG 2008 | DOI: 10.1002/cphc.200800210
High-speed AFM imaging of the heterodimeric FACT (facilitates chromatin transcription) protein reveals two tail-like structures protruding from its main body (see schematic picture). These very thin and undulating tail segments are identified as the intrinsically disordered regions of FACT predicted from the amino acid sequences, the mechanical properties of which, estimated from the AFM images, suggest that they have more relaxed structures than random coils.