Cryo-EM in the Study of Membrane Transport Proteins
Published Online: 1 JAN 2012
Copyright © 2011 American Physiological Society. All rights reserved.
How to Cite
Kim, L. Y., Johnson, M. C. and Schmidt-Krey, I. 2012. Cryo-EM in the Study of Membrane Transport Proteins. Comprehensive Physiology. 2:283–293.
- Published Online: 1 JAN 2012
Electron cryomicroscopy (cryo-EM) has evolved as a widely used approach to understand a range of structure-function questions, particularly of membrane proteins. Studies by both electron crystallography and single particle analysis have provided a wealth of information on membrane transport proteins. Cryo-EM methods with an emphasis on electron crystallography, which has yielded the most membrane transport protein structural information of any of the cryo-EM techniques, are described here. Two-dimensional crystallization approaches are outlined, as well as advances in cryo-EM specimen preparation, data collection, and image processing. Examples of membrane transport protein structure described serve to illustrate some of the advances in both structural understanding and methods. Further examples outline impressive results that were obtained by a combination of electron crystallography and X-ray crystallography as well as additional complementary methods. © 2012 American Physiological Society. Compr Physiol 2:283-293, 2012.