Standard Article

Release of free fatty acids from adipose tissue in vitro in relation to rates of triglyceride synthesis and degradation

Handbook of Physiology, Adipose Tissue

  1. Daniel Steinberg,
  2. Martha Vaughan

Published Online: 1 JAN 2011

DOI: 10.1002/cphy.cp050134

Comprehensive Physiology

Comprehensive Physiology

How to Cite

Steinberg, D. and Vaughan, M. 2011. Release of free fatty acids from adipose tissue in vitro in relation to rates of triglyceride synthesis and degradation. Comprehensive Physiology. 335–347.

Author Information

  1. National Heart Institute, National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 JAN 2011

Abstract

The sections in this article are:

  • 1
    I. Introduction
  • 2
    II. Measurement of the Rate of Triglyceride Breakdown: Methods and their Limitations
    • 2.1
      A. Free Fatty Acid Release
    • 2.2
      B. Glycerol Release
  • 3
    III. Measurement of the Rate of Triglyceride Synthesis: Methods and their Limitations
    • 3.1
      A. Incorporation of Labeled Glucose
    • 3.2
      B. Incorporation of Labeled Acetate
    • 3.3
      C. Incorporation of Labeled FFA
  • 4
    IV. Simultaneous Estimation of Rates of Triglyceride Breakdown and Synthesis: A Nonisotopic “Balance Method”
    • 4.1
      A. Net Glycerol Formation Reflects Exclusively Triglyceride Breakdown
    • 4.2
      B. Net FFA Formation Reflects Exclusively the Balance Between Formation and Breakdown of Ester Forms
  • 5
    V. Factors Influencing FFA Release, Triglyceride Breakdown and Triglyceride Synthesis
    • 5.1
      A. Normal Values
    • 5.2
      B. Metabolic Regulation
    • 5.3
      C. Direct Hormonal Regulation
    • 5.4
      D. Indirect Hormonal Control
    • 5.5
      E. Neural and Neurohumoral Control3