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Use of Cultured Cell Lines in Studies of Intestinal Cell Differentiation and Function

Handbook of Physiology, The Gastrointestinal System, Intestinal Absorption and Secretion

  1. Alain Zweibaum1,
  2. Marc Laburthe1,
  3. Etienne Grasset2,
  4. Daniel Louvard3

Published Online: 1 JAN 2011

DOI: 10.1002/cphy.cp060407

Comprehensive Physiology

Comprehensive Physiology

How to Cite

Zweibaum, A., Laburthe, M., Grasset, E. and Louvard, D. 2011. Use of Cultured Cell Lines in Studies of Intestinal Cell Differentiation and Function. Comprehensive Physiology. 223–255.

Author Information

  1. 1

    Unité de Recherches sur le Métabolisme et la Différenciation de Cellules en Culture, Institut National de la Santé et de la Recherche Médicale, Villejuif, France

  2. 2

    Service de Pédiatrie, Hôspital Hérold, Paris, France

  3. 3

    Unité de Biologie des Membranes, Département de Biologie Moléculaire, Institut Pasteur, Paris, France

Publication History

  1. Published Online: 1 JAN 2011

Abstract

The sections in this article are:

  • 1
    Cellular Models
    • 1.1
      Cell Lines From Normal Tissues
    • 1.2
      Cell Lines From Chemically Induced Tumors
    • 1.3
      Human Colon Carcinoma Cell Lines
  • 2
    Use of Cultured Cells for Study of Enterocytic Differentiation and Polarization
    • 2.1
      Polarized Organization of Enterocytes: Current Knowledge and Limitations
    • 2.2
      HT-29 and Caco-2 Cells as in Vitro Models: Advantages and Limitations
    • 2.3
      What Can We Learn From HT-29 and Caco-2 Cells?
  • 3
    Use of Cultured Cells for Study of Neurohormonal Receptors
    • 3.1
      Expression of Neurohormonal Receptors: Normal Intestine Versus Cell Line
    • 3.2
      Receptor Studies
  • 4
    Use of Cultured Cells for Study of Intestinal Transport
    • 4.1
      Transport Properties of Cultured Intestinal Cells
    • 4.2
      Regulation of Intestinal Transport: Effect of Secretagogues
    • 4.3
      Comparison With Other Existing Models: Possible Future Developments
  • 5
    Use of Cultured Cells for Study of Metabolic Functions
    • 5.1
      Colonic Malignant Cells as Models for Study of Glucose Metabolism
    • 5.2
      HT-29 Glucose-Negative Cells: Model for Study of Gluconeogenesis
  • 6
    Potential Use of Cultured Cells for Other Studies
  • 7
    Conclusion