Standard Article

Renal Micropuncture Techniques

Handbook of Physiology, Renal Physiology

  1. Heino Velazquez,
  2. Fred S. Wright

Published Online: 1 JAN 2011

DOI: 10.1002/cphy.cp080106

Comprehensive Physiology

Comprehensive Physiology

How to Cite

Velazquez, H. and Wright, F. S. 2011. Renal Micropuncture Techniques. Comprehensive Physiology. 249–269.

Author Information

  1. VA Medical Center, West Haven, Connecticut, and Departments of Internal Medicine and Cellular & Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut

Publication History

  1. Published Online: 1 JAN 2011

Abstract

The sections in this article are:

  • 1
    Animal Subjects
  • 2
    Equipment, Tools
    • 2.1
      Dissecting Microscopes
    • 2.2
      Compound Microscopes
    • 2.3
      Pipette Pullers and Grinders
    • 2.4
      Capillary Glass, Micropipettes
    • 2.5
      Syringes, Tubing
    • 2.6
      Micromanipulators
    • 2.7
      Microperfusion Pumps
    • 2.8
      Infusion Pumps
    • 2.9
      Heated Micropuncture Tables
    • 2.10
      Pressure Transducers
    • 2.11
      Specialized Surgical Equipment
    • 2.12
      Scintillation Counters
    • 2.13
      Voltmeters
    • 2.14
      Electrodes
    • 2.15
      Recorders
    • 2.16
      Oil and Wax Blocks
    • 2.17
      Wax Injection Apparatus
    • 2.18
      Timers
  • 3
    In Vivo Micropuncture: Functional Assessment of Individual Nephron Segments
    • 3.1
      Anesthesia and Surgery
    • 3.2
      Evaluation of Kidney before Micropuncture
    • 3.3
      Micropipette Placement
    • 3.4
      Identification of Accessible Cortical Tubules and Vessels
    • 3.5
      Identification of Papillary Structures
    • 3.6
      Measurement of Distance to Puncture Site and Length of Perfused Tubule
    • 3.7
      Determination of Filtration and Transport Rates
    • 3.8
      Determination of Blood Constituents
    • 3.9
      Measurement of Capillary and Tubule Pressure
    • 3.10
      Potential Problems
  • 4
    Micropuncture in Nonstandard Preparations
  • 5
    Microanalytical Techniques
    • 5.1
      Volume
    • 5.2
      Inulin
    • 5.3
      Protein
    • 5.4
      Urea
    • 5.5
      Osmolality
    • 5.6
      Metal Cations
    • 5.7
      Halide Anions
    • 5.8
      Ammonia
    • 5.9
      Carbon Dioxide and Bicarbonate