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Renal Plasma Membranes: Isolation, General Properties, and Biochemical Components

Handbook of Physiology, Renal Physiology

  1. Rolf Kinne,
  2. E. Kinne-Saffran

Published Online: 1 JAN 2011

DOI: 10.1002/cphy.cp080245

Comprehensive Physiology

Comprehensive Physiology

How to Cite

Kinne, R. and Kinne-Saffran, E. 2011. Renal Plasma Membranes: Isolation, General Properties, and Biochemical Components. Comprehensive Physiology. 2083–2117.

Author Information

  1. Max-Planck-Institut für Systemphysiologie, Dortmund, Germany

Publication History

  1. Published Online: 1 JAN 2011

Abstract

The sections in this article are:

  • 1
    Studies on Isolated Plasma Membranes: Conceptual Framework
  • 2
    Isolation of Plasma Membranes of the Proximal Tubule
    • 2.1
      Definition of Membranes
    • 2.2
      Membrane Marker
    • 2.3
      Starting Material
    • 2.4
      Isolation of Microvillous Membranes
    • 2.5
      Isolation of Basal-lateral Membranes
    • 2.6
      Simultaneous Isolation of Microvillous and Basal-lateral Membranes
    • 2.7
      Isolation of Endosomes and Reserve Vesicles
  • 3
    Purity and General Properties of Plasma Membranes Isolated from the Proximal Tubule
    • 3.1
      Microvillous Membranes
    • 3.2
      Basal-lateral Membranes
    • 3.3
      Endosomes and Reserve Vesicles
  • 4
    Isolation of Plasma Membranes of the Thick Ascending limb of Henle's Loop
    • 4.1
      Definition of Membranes
    • 4.2
      Membrane Marker
    • 4.3
      Starting Material
    • 4.4
      Methods for Membrane Isolation
  • 5
    Purity and General Properties of Plasma Membranes Isolated from the Thick Ascending Limb
    • 5.1
      Purity of Membrane Fractions
    • 5.2
      General Properties
  • 6
    Isolation of Plasma Membranes of the Collecting Duct
    • 6.1
      Definition of Membranes
    • 6.2
      Membrane Marker
    • 6.3
      Starting Material
    • 6.4
      Methods for Membrane Isolation
  • 7
    Purity and General Properties of Membranes Isolated from the Collecting Duct
  • 8
    Biochemical Components of Isolated Renal Plasma Membranes
    • 8.1
      Membrane Lipids
    • 8.2
      Lipid Composition of Isolated Membranes of the Proximal Tubule
    • 8.3
      Lipid Composition of Plasma Membranes Isolated from Kidney Medulla
    • 8.4
      Identification of Membrane Proteins
    • 8.5
      Enzymes in Renal Membranes
    • 8.6
      Antigens in Renal Membranes
    • 8.7
      Transport Proteins in Renal Membranes
    • 8.8
      Protein Fingerprints of Renal Membranes
  • 9
    Concluding Remarks