Standard Article

Membrane Transport in Single Cells

Handbook of Physiology, Cell Physiology

  1. Michael L. Jennings1,
  2. Mark A. Milanick2

Published Online: 1 JAN 2011

DOI: 10.1002/cphy.cp140107

Comprehensive Physiology

Comprehensive Physiology

How to Cite

Jennings, M. L. and Milanick, M. A. 2011. Membrane Transport in Single Cells. Comprehensive Physiology. 261–308.

Author Information

  1. 1

    Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas

  2. 2

    Department of Physiology, University of Missouri School of Medicine, and Dalton Cardiovascular Research Center, Columbia, Missouri

Publication History

  1. Published Online: 1 JAN 2011

Abstract

The sections in this article are:

  • 1
    Examples of Preparations of Single Cells
    • 1.1
      Large Cells that Can Be Studied Individually
    • 1.2
      Blood Cells
    • 1.3
      Cells Prepared by Dissociating Intact Tissue
    • 1.4
      Permanent Cell Lines and Expression Systems
  • 2
    Methods for Measuring Transport
    • 2.1
      Tracer Influx and Efflux: Definition of Flux
    • 2.2
      Definition of Permeability Coefficient
    • 2.3
      Unstirred Layers
    • 2.4
      Multiple Intracellular Compartments
    • 2.5
      Optical Methods for Measuring Transport
    • 2.6
      Ion-Sensitive Microelectrodes
    • 2.7
      Membrane Potential
    • 2.8
      Nuclear Magnetic Resonance
  • 3
    Transport Mechanisms
    • 3.1
      Rationale for Investigating Transport Mechanisms
    • 3.2
      Catalytic Cycles
  • 4
    Anion Exchanger
    • 4.1
      Red Blood Cell Anion Transport
    • 4.2
      Electrically Silent Obligatory Exchange
    • 4.3
      Kinetic Mechanism of Anion Exchange
    • 4.4
      Intrinsic Asymmetries
    • 4.5
      Electrical Properties of the Exchange Pathway
  • 5
    Sodium Pump
    • 5.1
      Electrically Silent and Electrogenic Na Pump Modes
    • 5.2
      Pump Stoichiometry
    • 5.3
      Kinetic Mechanism of the Na Pump
    • 5.4
      Electrical Properties of the Na Pump
    • 5.5
      Summary of Transport Mechanisms
  • 6
    Integrative Transport Studies in Single Cells: Cell-Volume Regulation
    • 6.1
      Pump-Leak Paradigm
    • 6.2
      Volume-Sensitive Transport Processes
    • 6.3
      Detection of Cell Volume
    • 6.4
      Transduction of Volume Signal to Transport Proteins