Trypsin-Immobilized Metal–Organic Framework as a Biocatalyst In Proteomics Analysis

Authors

  • Yung-Han Shih,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Sheng-Han Lo,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Ni-Shin Yang,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Brenda Singco,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Yi-Jie Cheng,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Cheng-You Wu,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • I-Hsin Chang,

    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Prof. Hsi-Ya Huang,

    Corresponding author
    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
    • Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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  • Prof. Chia-Her Lin

    Corresponding author
    1. Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
    • Department of Chemistry, Chung Yuan Christian University, 200 Chung Pei Road, Chung-Li 320 (Taiwan), Fax: (+886) 3-2653399
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Abstract

original image

Enzyme immobilization: The protease enzyme was successfully immobilized onto dicyclohexylcarbodiimide (DCC)-activated metal–organic frameworks (MOFs). After separation by nano-LC-MS2 (liquid chromatography–mass spectrometry), detection, and database searching the protein digestion efficiency of trypsin-MOF was comparable to traditional in-solution digestion (see figure). Furthermore, the trypsin-MOF was reusable.

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