Attachment of Proteins to Surfaces by Fluorous–Fluorous Interactions Restoring Their Structure and Activity

Authors

  • Hartmut M. Rapp,

    1. Albert-Ludwigs-University Freiburg i. Br. Department of Chemistry, Pharmacy and Earth Science, Institute of Organic Chemistry, Albertstrasse 21, 79104 Freiburg (Germany), Fax: (+49) 761-203-8705
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  • Stefanie Bacher,

    1. Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131 Karlsruhe (Germany)
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  • Anika Ahrens,

    1. Albert-Ludwigs-University Freiburg i. Br. Department of Chemistry, Pharmacy and Earth Science, Institute of Organic Chemistry, Albertstrasse 21, 79104 Freiburg (Germany), Fax: (+49) 761-203-8705
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  • Dr. Wolfgang Rapp,

    1. Rapp Polymere, Ernst-Simon-Strasse 9, 72072 Tübingen (Germany)
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  • Dr. Bernd Kammerer,

    1. Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg i.Br. Habsburgerstrasse 49, 79104 Freiburg (Germany)
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  • Prof. Dr. G. Ulrich Nienhaus,

    1. Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131 Karlsruhe (Germany)
    2. Department of Physics, University of Illinois Urbana-Champaign, Urbana, IL 61801 (USA)
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  • Prof. Dr. Willi Bannwarth

    Corresponding author
    1. Albert-Ludwigs-University Freiburg i. Br. Department of Chemistry, Pharmacy and Earth Science, Institute of Organic Chemistry, Albertstrasse 21, 79104 Freiburg (Germany), Fax: (+49) 761-203-8705
    • Albert-Ludwigs-University Freiburg i. Br. Department of Chemistry, Pharmacy and Earth Science, Institute of Organic Chemistry, Albertstrasse 21, 79104 Freiburg (Germany), Fax: (+49) 761-203-8705
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Abstract

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Above the fold: An innovative strategy using a multifunctional linker system for the specific noncovalent immobilization of proteins to glass surfaces through fluorous–fluorous interactions without causing significant denaturation has been achieved. The principle was demonstrated for the enzymes RNase H and recombinant horseradish peroxidase (see figure).

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