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Communication

Attachment of Proteins to Surfaces by Fluorous–Fluorous Interactions Restoring Their Structure and Activity

  1. Hartmut M. Rapp1,
  2. Stefanie Bacher2,
  3. Anika Ahrens1,
  4. Dr. Wolfgang Rapp3,
  5. Dr. Bernd Kammerer4,
  6. Prof. Dr. G. Ulrich Nienhaus2,5,
  7. Prof. Dr. Willi Bannwarth1,*

Article first published online: 8 NOV 2012

DOI: 10.1002/cplu.201200234

Issue

ChemPlusChem

ChemPlusChem

Volume 77, Issue 12, pages 1066–1070, December 2012

Additional Information

How to Cite

Rapp, H. M., Bacher, S., Ahrens, A., Rapp, W., Kammerer, B., Nienhaus, G. U. and Bannwarth, W. (2012), Attachment of Proteins to Surfaces by Fluorous–Fluorous Interactions Restoring Their Structure and Activity. ChemPlusChem, 77: 1066–1070. doi: 10.1002/cplu.201200234

Author Information

  1. 1

    Albert-Ludwigs-University Freiburg i. Br. Department of Chemistry, Pharmacy and Earth Science, Institute of Organic Chemistry, Albertstrasse 21, 79104 Freiburg (Germany), Fax: (+49) 761-203-8705

  2. 2

    Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131 Karlsruhe (Germany)

  3. 3

    Rapp Polymere, Ernst-Simon-Strasse 9, 72072 Tübingen (Germany)

  4. 4

    Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg i.Br. Habsburgerstrasse 49, 79104 Freiburg (Germany)

  5. 5

    Department of Physics, University of Illinois Urbana-Champaign, Urbana, IL 61801 (USA)

*Albert-Ludwigs-University Freiburg i. Br. Department of Chemistry, Pharmacy and Earth Science, Institute of Organic Chemistry, Albertstrasse 21, 79104 Freiburg (Germany), Fax: (+49) 761-203-8705

Publication History

  1. Issue published online: 13 DEC 2012
  2. Article first published online: 8 NOV 2012
  3. Manuscript Revised: 17 OCT 2012
  4. Manuscript Received: 7 SEP 2012

Funded by

  • Deutsche Forschungsgemeinschaft (DFG)
  • Center for Functional Nanostructures (CFN). Grant Number: NI 291/9

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Keywords:

  • fluorous–fluorous interactions;
  • FRET;
  • protein immobilization;
  • rcHRP;
  • RNase H
Thumbnail image of graphical abstract

Above the fold: An innovative strategy using a multifunctional linker system for the specific noncovalent immobilization of proteins to glass surfaces through fluorous–fluorous interactions without causing significant denaturation has been achieved. The principle was demonstrated for the enzymes RNase H and recombinant horseradish peroxidase (see figure).

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