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Trimethylation and Differential Mobility Spectroscopy in Quantitative Peptide Analysis: Increasing Selectivity and Sensitivity through Ion/Molecule Chemistry

Authors

  • Voislav Blagojevic,

    1. Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3 (Canada), Fax: (+1) 416-736-5936
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  • Diethard K. Bohme

    Corresponding author
    1. Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3 (Canada), Fax: (+1) 416-736-5936
    • Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3 (Canada), Fax: (+1) 416-736-5936
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Abstract

Isotopic labeling of peptides by trimethylation creates a charged quaternary amine group on the peptide that provides clear differentiation from unlabeled protonated peptides and protonated or sodiated chemical background. Differential mobility spectrometry, with its use of a chemical modifier, allows otherwise undesirable ion/molecule reactions in the mobility cell to increase selectivity and sensitivity of quantitative peptide analysis. A high proton affinity modifier selectively removes protonated and sodiated interference and background ions by proton and sodium transfer, while leaving the trimethylated ions with their quaternary amine groups unchanged.

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