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Synthesis of Polyboronic Acid Functionalized Hierarchically Ordered Macro-/Mesoporous Silica for Selective Enrichment of Glycopeptides for Mass Spectrometric Analysis

Authors

  • Dr. Yinghua Yan,

    1. Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433 (P. R. China), Fax: (+86) 21-65641740
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  • Prof. Chunhui Deng,

    Corresponding author
    1. Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433 (P. R. China), Fax: (+86) 21-65641740
    • Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433 (P. R. China), Fax: (+86) 21-65641740

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  • Zhifang Zheng,

    1. Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433 (P. R. China), Fax: (+86) 21-65641740
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  • Prof. Xiangmin Zhang,

    1. Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433 (P. R. China), Fax: (+86) 21-65641740
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  • Prof. Pengyuan Yang

    1. Department of Chemistry and Institutes of Biomedical Science, Fudan University, Shanghai 200433 (P. R. China), Fax: (+86) 21-65641740
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Abstract

A novel hierarchically ordered macro/mesoporous silica was functionalized with polyboronic acid (HOMMS@PolyVPBA). This composite possesses the attractive features of high surface area and large accessible porosity, and was applied to enrich glycopeptides. Here the HOMMS material was introduced into glycoproteome for the first time. The HOMMS@PolyVPBA gave remarkable selectivity for glycopeptides even at a low molar ratio of glycopeptides/nonglycopeptides (1:50) with a very rapid enrichment speed (only 10 min) and low detection (1 ng μL−1). Therefore the synthetic HOMMS@PolyVPBA might be a promising selective enrichment material for glycoproteome analysis.

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