Titanium(IV)-Immobilized Hydrophilic Hierarchically Ordered Macro-/Mesoporous Silica for Fast Enrichment of Phosphopeptides

Authors

  • Dr. Yinghua Yan,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (P. R. China)
    Search for more papers by this author
  • Zhifang Zheng,

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (P. R. China)
    Search for more papers by this author
  • Yan Li,

    Corresponding author
    1. Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203 (P. R. China)
    • Yan Li, Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203 (P. R. China)

      Chunhui Deng, Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (P. R. China)

    Search for more papers by this author
  • Prof. Chunhui Deng,

    Corresponding author
    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (P. R. China)
    • Yan Li, Pharmaceutical Analysis Department, School of Pharmacy, Fudan University, Shanghai 201203 (P. R. China)

      Chunhui Deng, Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (P. R. China)

    Search for more papers by this author
  • Prof. Xiangmin Zhang

    1. Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433 (P. R. China)
    Search for more papers by this author

Abstract

Feasible design is essential to achieve ideal chemical and biological properties of nanomaterials. For the first time, new nanocomposites with a polydopamine coating on hierarchically ordered macro-/mesoporous silica functionalized with titanium ions (denoted as HOMMS-PD-Ti4+) were prepared through a facile reaction route at room temperature. The applicability of as-synthesized HOMMS-PD-Ti4+ for the selective enrichment of phosphopeptides was tested. The experimental results demonstrate that, by taking advantage of the pure phosphate–Ti4+ interface and high loading amount of Ti4+, HOMMS-PD-Ti4+ presents remarkable selectivity for phosphopeptides, even at a low molar ratio of phosphopeptides/non-phosphopeptides (1:1000) with a very rapid enrichment speed (within 1 min). The superior sensitivity for low-abundant phosphopeptides and the high selectivity and effectiveness for the enrichment of phosphopeptides from human serum are also proven. These outstanding features demonstrate that HOMMS-PD-Ti4+ exhibits great potential in phosphoproteome research in the future.

Ancillary