A Probe for the Selective and Parts-per-Billion-Level Detection of Copper(II) and Mercury(II) using a Micellar Medium and Its Utility in Cell Imaging

Authors

  • Dr. Namita Kumari,

    1. Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012 1 (India), Fax: (+91) 080-2360-0529
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  • Dr. Satadru Jha,

    1. Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012 1 (India), Fax: (+91) 080-2360-0529
    2. Department of Chemistry, Sikkim Manipal Institute of Technology, Majitar, East-Sikkim 737136 (India)
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  • Dr. Santosh K. Misra,

    1. Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012 1 (India), Fax: (+91) 080-2360-0529
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  • Prof. Dr. Santanu Bhattacharya

    Corresponding author
    1. Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012 1 (India), Fax: (+91) 080-2360-0529
    2. Chemical Biology Unit, Jawaharlal Nehru Centre of Advanced Scientific Research, Bangalore, Karnataka 560064 (India)
    • Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012 1 (India), Fax: (+91) 080-2360-0529

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Abstract

A novel colorimetric probe 1 based on the picolyl moiety has been designed and synthesized. Probe 1 is composed of a pyrene and a bispicolyl amine (BPA) unit, in which the BPA moiety acts as a binding unit and the binding phenomenon is sensed from the changes in the signaling subunit. The probe detects Cu2+ specifically in water and both Cu2+ and Hg2+ efficiently in neutral Brij-58 micellar media. The probe shows a color change visible to the naked eye upon addition of metal ions. Notably, in a micellar medium, probe 1 can detect both the Cu2+ and Hg2+ ions even at parts-per-billion levels. Furthermore, the probe shows ratiometric detection of both the metal ions making the sensing quantitative. The two metal ions could be discriminated both visibly under a UV lamp and with the use of fluorescence spectroscopy. The probe could be also used in biological cell lines for the detection of both Hg2+ and Cu2+ ions.

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