Probing Wavelength Regulation with an Engineered Rhodopsin Mimic and a C15-Retinal Analogue (pages 273–276)
Dr. Kin Sing S. Lee, Tetyana Berbasova, Dr. Chrysoula Vasileiou, Dr. Xiaofei Jia, Dr. Wenjing Wang, Yoomi Choi, Farid Nossoni, Prof. James H. Geiger and Prof. Babak Borhan
Article first published online: 29 FEB 2012 | DOI: 10.1002/cplu.201100082
Shield and control: The application of a protein design strategy to interrogate the perception of color is described. Cellular retinoic acid binding protein II (see structure) was used first to show that complete encapsulation of the chromophore is critical to achieve an opsin shift comparable to the one observed for rod rhodopsin, and second to show that a rhodopsin protein mimic capable of binding a retinoid analogue responds to changes in electrostatic environments.